The goal of this study is to reveal the characters and therapeutic targets of CNS leukemia.
No associated publication
Sex, Age, Specimen part, Disease
View SamplesBuilding the gene expression profiles and identifying the differentially expressed genes in specific comparisons.
No associated publication
No sample metadata fields
View SamplesRNA-seq of single embryo
No associated publication
No sample metadata fields
View SamplesVarious pluripotent stem (PS) cells can be isolated from early developing embryos in mouse. Among these, two kinds of PS cells were isolated from mouse blastocysts: conventional embryonic stem (ES) cells with domed morphology that are maintained with LIF and BMP for self-renewal, and FAB-ES cells with flat morphology that need bFGF, activinA and BIO for self-renewal. Here, we report a novel PS cell line from rat blastocysts, which is distinguishable from conventional ES cells but is morphologically similar to mouse epiblast stem cell (EpiSC) lines. We used microarrays to detail the global program of gene expression of rES and rPS.
The heterogeneity and dynamic equilibrium of rat embryonic stem cells.
Specimen part
View SamplesNIH-3T3 cells transduced with either EBF1-, PPARg2- or empty vector were stimulated with hormones to initiate adipocyte differentiation. RNA extraction was done using TriZol at d0, d2, d4 and d10 after stimulation. Samples were handled according to standard affymetrix protocols.
Gene expression analysis suggests that EBF-1 and PPARgamma2 induce adipogenesis of NIH-3T3 cells with similar efficiency and kinetics.
No sample metadata fields
View SamplesNPTX1 is a key inducer of neural lineages from the human ESC.
NPTX1 regulates neural lineage specification from human pluripotent stem cells.
Cell line, Time
View SamplesPurpose:The goals of this study are to understand the mechanisms underlying reduced self-renewal and loss of pluripotency by depletion of Rif1.
Rif1 maintains telomere length homeostasis of ESCs by mediating heterochromatin silencing.
Cell line
View SamplesNicotinamide (NAM) inhibited the expression of Age related macular degeneration (AMD) associated proteins in hiPSC-derived retinal pigment epithelium (RPE).
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Disease, Treatment
View SamplesRNA-Seq analysis was performed to identify differentially expressed transcripts in primary AML bone marrow derived mononuclear cells compared to healthy (normal) peripheral blood mononuclear cells.
No associated publication
Sex, Age, Specimen part, Disease, Disease stage, Race
View SamplesWe have used lentiviral mediated knock down of SMARCB1, a subunit of the mammalian SWI/SNF complex in HL60, an acute promyelocytic leukemia cell line followed by high throughput mRNA sequencing to identify differentially expressed genes. We have used this data to identify genes affected by SMARCB1 depletion, and thus understand contribution of SMARCB1 towards leukemogenesis.
No associated publication
Sex, Age, Specimen part, Cell line
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