CHD3 proteins are ATP-dependent chromatin remodeling factors that are components of diverse multisubunit complexes that can either repress or activate gene expression. In plants, the CHD3 protein PICKLE (PKL) is necessary for repression of seed-specific genes during germination and promotes deposition of the repressive epigenetic mark trimethylation of histone H3 lysine 27 (H3K27me3). It is unknown, however, if PKL acts directly at H3K27me3-enriched loci. We undertook a microarray analysis of 14-day-old plants and found that PKL continues to play an important role in expression of H3K27me3-enriched genes and in specification of developmental identity after germination.
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Specimen part
View SamplesMicroarrays revolutionized biological research by enabling gene expression comparisons on a transcriptome-wide scale. Microarrays, however, do not estimate absolute expression level accurately. At present, high throughput sequencing is emerging as an alternative methodology for transcriptome studies. Although free of many limitations imposed by microarray design, its potential to estimate absolute transcript levels is unknown.
Estimating accuracy of RNA-Seq and microarrays with proteomics.
Sex, Age
View Samplesto identify regulated genes in response to cytokinin in wild-type and 35S:ARR7 plants using the Affymetrix ATH1 full genome array.
Genome-wide expression profiling of ARABIDOPSIS RESPONSE REGULATOR 7(ARR7) overexpression in cytokinin response.
Disease, Disease stage, Compound, Time
View SamplesThe Columbia (Col-0) ecotype of Arabidopsis thaliana was used as wild type. lbd16, lbd18 single and lbd16 lbd18 double mutants were used as mutants.
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Time
View SamplesThe experiment was designed to enable comparison between Columbia and ahk2/ahk3, ahk3/ahk4 double and ahk2/ahk3/ahk4 triple mutants Arabidopsis seedlings
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Age, Time
View SamplesThe iaa1 mutant protein impaired a variety of auxin responses by acting as a negative regulator of auxin-responsive pathway.
Genome-wide analysis of the auxin-responsive transcriptome downstream of iaa1 and its expression analysis reveal the diversity and complexity of auxin-regulated gene expression.
Age, Compound, Time
View SamplesTo decipher the function of SUA5, we compare the expression profiles of sua5D and wildtype BY4742. We also compared the expression profile changed during the passage of sua5D strain.
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No sample metadata fields
View SamplesTime-course analysis of shade responsive genes in Col and 12 mutants.
No associated publication
Specimen part, Treatment
View SamplesTime-course data of shade avoidance in NAM parents
No associated publication
Specimen part, Treatment
View SamplesDevelopmental gradient of expanding maize leaf
No associated publication
Age, Specimen part
View Samples