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Arabidopsis thaliana
36 Downloadable Samples
Illumina HiSeq 2000
Description
Cold stress response is extensively studied process in plants. However most studies explore only limited set of organs and developmental stages (leaves or seedlings). In order to gain insight into organ-specific strategies of cold stress response we studied expression changes that follow exposure to cold (+4ºC) in different aerial parts of plant: cotyledons, hypocotyl, leaves, young flowers, mature flowers and seeds using RNA-seq. It showed that gene expression is highly organ-specific. The results on differential expression in leaves are congruent with current knowledge on stress response pathways, in particular, the role of CBF genes. In other organs, both essence and dynamics of gene expression changes are different. We show the involvement of genes that are confined to narrow expression patterns in non-stress conditions into stress response. In particular, the genes that control cell wall modification in pollen, are activated in leaves. In seeds, predominant pattern is the change of lipid metabolism. We found that stress response is highly organ-specific and highlighted the processes that are involved in this process in each type of organs.
Publication Title
No associated publication
Sample Metadata Fields
Specimen part
View Samples
SRP050879- Danio rerio
- 1 Downloadable Sample
- IlluminaHiSeq2000
Description
we aimed to calculate the expression level of some special genes
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples- Drosophila melanogaster
- 6 Downloadable Samples
- Illumina HiSeq 2000
Description
Early embryo RNA-seq sequencing
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part
View Samples SRP090913- Arabidopsis thaliana
- 12 Downloadable Samples
- Illumina HiSeq 3000
Description
Cell wall modifications in response to low temperature compensation
Publication Title
No associated publication
Sample Metadata Fields
Specimen part, Treatment
View Samples- Homo sapiens
- 4 Downloadable Samples
- Illumina HiSeq 2000
Description
In this study, we purified periodontal ligament cell clones committed to osteoblastic/cementoblastic phenotype (C-O clones) and clones committed to fibroblastic phenotype (C-F clones), and employed RNA-seq to describe the differential transcriptional profile of osteoblastic/cementoblastic and fibroblastic cell clones from human periodontal ligament. The understanding of differences in periodontal ligament subpopulations can help to elucitade the favorable to formation of mineralizing and non-mineralizing tissues of periodontium.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Age, Specimen part
View Samples- Homo sapiens
- 4 Downloadable Samples
- Illumina HiSeq 2000
Description
In this study, we purified periodontal ligament cell clones committed to osteoblastic/cementoblastic phenotype (C-O clones) and clones committed to fibroblastic phenotype (C-F clones), and employed RNA-seq to describe the differential transcriptional profile of osteoblastic/cementoblastic and fibroblastic cell clones from human periodontal ligament. The understanding of differences in periodontal ligament subpopulations can help to elucitade the favorable to formation of mineralizing and non-mineralizing tissues of periodontium.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Age, Specimen part, Treatment
View Samples- Mus musculus
- 45 Downloadable Samples
Illumina MouseWG-6 v2.0 expression beadchip
Description
This SuperSeries is composed of the SubSeries listed below.
Publication Title
The Methyl-CpG-Binding Protein Mbd2 Regulates Susceptibility to Experimental Colitis via Control of CD11c<sup>+</sup> Cells and Colonic Epithelium.
Sample Metadata Fields
Specimen part
View Samples- Mus musculus
- 27 Downloadable Samples
Illumina MouseWG-6 v2.0 expression beadchip
Description
Mice deficient in MBD2 (Mbd2-/-) were treated with 2% dextran sulfate sodium or normal drinking water for 6 continuous days. A single cell suspension of colon lamina propria and epithelium was isolated, with monocytes (CD11b+ Ly6CHi, MHC-II+/-), macrophages (CD11b+ Ly6C-MHC-II+), cDC2s (CD11b- CD11c+ CD103+) and epithelial cells (CD45- EpCAM+) purified by FACS.
Publication Title
The Methyl-CpG-Binding Protein Mbd2 Regulates Susceptibility to Experimental Colitis via Control of CD11c<sup>+</sup> Cells and Colonic Epithelium.
Sample Metadata Fields
Specimen part
View Samples- Mus musculus
- 18 Downloadable Samples
- Affymetrix Mouse Gene 1.1 ST Array (mogene11st)
Description
T follicular helper cells (TFH) are heterogenic population of CD4+ T cells, expressing CXCR5+ and PD-1+ on their surface. Their role is linked to supporting formation of germinal centres (GC) and these cells are thought to express high levels of PD-1 marker. Two models of immunisation were used to investigate the role of PD-1 low TFH. In Salmonella enterica infection high frequency of T follicular helper cells expressing low levels of PD-1 surface molecule are observed within first week of infection but GC do not appear until much a later stage (week 7-8). Sheep red blood cell immunisation (SRBC) gives rise to both TFH and GC B cells within first week of response and these TFH express low to high level of PD-1 molecule.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part
View Samples- Mus musculus
- 18 Downloadable Samples
- Illumina MouseWG-6 v2.0 expression beadchip
Description
Mice deficient in MBD2 (Mbd2-/-) were treated with 2% dextran sulfate sodium or normal drinking water for 6 continuous days. A single cell suspension of colon lamina propria and epithelium was isolated, with monocytes (CD11b+ Ly6CHi, MHC-II+/-), macrophages (CD11b+ Ly6C-MHC-II+), cDC2s (CD11b- CD11c+ CD103+) and epithelial cells (CD45- EpCAM+) purified by FACS.
Publication Title
The Methyl-CpG-Binding Protein Mbd2 Regulates Susceptibility to Experimental Colitis via Control of CD11c<sup>+</sup> Cells and Colonic Epithelium.
Sample Metadata Fields
Specimen part
View Samples