Filters
Technology
Platform
Zea mays
5 Downloadable Samples
Illumina HiSeq 2500
Description
The Mechanisms of Maize Resistance to Fusarium verticillioides by comprehensive analysis of RNA-seq Data
Publication Title
No associated publication
Sample Metadata Fields
Age, Specimen part, Disease, Disease stage, Treatment
View Samples
SRP132125- Zea mays
- 7 Downloadable Samples
- Illumina HiSeq 2500
Description
To study the splicing factor role of ZmGRP1
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part, Disease, Disease stage, Cell line, Treatment
View Samples- Danio rerio
- 5 Downloadable Samples
- IlluminaHiSeq2500
Description
A genome-wide map of circular RNA in adult zebrafish.
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples- Mus musculus
- 4 Downloadable Samples
- Illumina HiSeq 2000
Description
Spermatogenesis is a complex differentiation process that involves the successive and simultaneous execution of three different gene expression programs: mitotic proliferation of spermatogonia, meiosis, and spermiogenesis. Testicular cell heterogeneity has hindered its molecular analyses. Moreover, the characterization of short, poorly represented cell stages such as initial meiotic prophase ones (leptotene and zygotene) has remained elusive, despite their crucial importance for understanding the fundamentals of meiosis.We have developed a flow cytometry-based approach for obtaining highly pure stage-specific spermatogenic cell populations, including early meiotic prophase. Here we combined this methodology with next generation sequencing, which enabled the analysis of meiotic and postmeiotic gene expression signatures in mouse with unprecedented reliability. Interestingly, we found that a considerable number of genes involved in early as well as late meiotic processes are already on at early meiotic prophase, with a high proportion of them being expressed only for the short time lapse of lepto-zygotene stages. Besides, we observed a massive change in gene expressionpatterns during medium meiotic prophase (pachytene) when mostly genes related to spermiogenesis and sperm function are already turned on. This indicates that the transcriptional switch from meiosis to post-meiosis takes place very early, during meiotic prophase, thus disclosing a higher incidence of post-transcriptional regulationin spermatogenesis than previously reported. Moreover, we found that a good proportion of the differential gene expression in spermiogenesis corresponds to up-regulation of genes whose expression starts earlier, at pachytene stage; this includes transition protein- and protamine-coding genes, which have long been claimed to switch on during spermiogenesis. In addition, our results afford new insights concerning X chromosome meiotic inactivation and reactivation. This work provides for the first time an overview of the time course for the massive onset and turning off of the meiotic and spermiogenic genetic programs. Importantly, our data represent a highly reliable information set about gene expression in pure testicular cell populations including early meiotic prophase, for further data mining towards the elucidation of the molecular bases of male reproduction in mammals.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part, Cell line
View Samples- Sus scrofa
- 3 Downloadable Samples
- Illumina Genome Analyzer IIx
Description
Pig RNA-seq.
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples- Arabidopsis thaliana
- 2 Downloadable Samples
- Illumina HiSeq 2000
Description
Transcriptomic analysis of BnLATE-regulated pathways to investigate the genome-wide effects of BnLATE overexpression on transcription
Publication Title
No associated publication
Sample Metadata Fields
Specimen part
View Samples SRP082943- Zea mays
- 72 Downloadable Samples
- Illumina HiSeq 2000
Description
To reveal the dynamic expression of genes involved in PPDK regulation, different leaf sections of PPDK mutant, wild type and its heterozygous, which were grown under high and low light, were subjected to RNA-seq sequencing.
Publication Title
No associated publication
Sample Metadata Fields
Specimen part
View Samples GSE2515- Mus musculus
- 6 Downloadable Samples
Affymetrix Mouse Expression 430A Array (moe430a)
Description
TEC (B6 vs Aire KO). Thymic epithelial cells from B6 strain and AIRE KO are compared.
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples- Homo sapiens
- 1 Downloadable Sample
Description
We propose that the stochastic combination of alternative transcription events has contributed to complex isoform evolution.
Publication Title
Evolutionary interrogation of human biology in well-annotated genomic framework of rhesus macaque.
Sample Metadata Fields
No sample metadata fields
View Samples- Gallus gallus
- 60 Downloadable Samples
- Affymetrix Chicken Gene 1.0 ST Array (chigene10st)
Description
We used a chicken RNA microarray to identify differentially expressed genes in order to compare two layer lines kept in a small group housing system Eurovent Deutsch.
Publication Title
No associated publication
Sample Metadata Fields
Specimen part
View Samples