To provide novel insights into the molecular basis of floral initiation, RNASeq was used to characterize the soybean transcriptome of leaf and micro-dissected shoot apical meristem at different time points after short-day treatment.
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View SamplesPowdery mildew, caused by the fungus Blumeria graminis (DC) Speer, is one of the most important foliar diseases of cereals worldwide. It is an obligate biotrophic parasite, colonising leaf epidermal cells to obtain nutrients from the plant cells without killing them. Syringolin A (sylA), a circular peptide secreted by the phytopathogenic bacterium Pseudomonas syringae pv. syringae, triggers a hypersensitive cell death reaction (HR) at infection sites when sprayed onto powdery mildew infected wheat which essentially eradicates the fungus. The rational was to identify genes whose expression was specifically regulated during HR, i.e. genes that might be involved in the switch of compatibility to incompatibility.<br></br>Powdery mildew-infected or uninfected plants were treated with syringolin two days after infection and plant material for RNA extraction was collected at 0.5, 1, 2, 4, 8, 12 hours after treatment (hat), resulting in an early (2 and 4 hat) and late pool (8 and 12 hat). Plant material that was uninfected prior to syringolin treatment was collected 8 and 12 hat (late pool of uninfected plant material), and 1 hat, respectively.
Transcriptional changes in powdery mildew infected wheat and Arabidopsis leaves undergoing syringolin-triggered hypersensitive cell death at infection sites.
Compound, Time
View SamplesAlthough it is well known that the function of MyoG cannot be compensated for other MRFs, the molecular mechanism by which MyoG controls muscle cell differentiation is still unclear. Therefore, in this study, RNA-Seq technology was applied to profile changes in gene expression in response to MyoG knock-down (MyoGkd) in primary bovine muscle satellite cells (MSCs). This study is the first RNA-Seq based gene expression analysis of MyoGkd undertaken in primary bovine MSCs.
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Sex, Specimen part, Disease
View SamplesRNA-Seq analysis of the drought responsive transcriptome of Zea mays cultivar Z59
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Specimen part, Treatment
View SamplesTime-course analysis of shade responsive genes in Col and 12 mutants.
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Specimen part, Treatment
View SamplesTime-course data of shade avoidance in NAM parents
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Specimen part, Treatment
View SamplesDevelopmental gradient of expanding maize leaf
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Age, Specimen part
View SamplesIn order to study the gene expression of pollen tubes as they grow in silk after pollination, we pollinated maize W22 silks with maize B73 pollen. The recent (2016) advent of the W22 genome assembly and annotation allows us to single out RNA-seq reads originating from the pollen tubes. B73 pollen, W22 silk and B73 seedling controls were sequenced as well.
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Specimen part
View SamplesTotal RNA of thymus from mouse different development stages were isolated and deeply sequenced by SAPAS to investigated the global gene regualtion events during thymus genesis and maturation.
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View SamplesRectal cancer stage II carcinoma and adjacent normal tissue transcriptome
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