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SRP014772
Danio rerio
12 Downloadable Samples
IlluminaHiSeq2000
Description
Forward genetic screens have elucidated molecular pathways required for innumerable aspects of life, however identifying the causal mutations from such screens has long been the bottleneck in the process, particularly in vertebrates. We have developed an RNA-Seq based approach that identifies both the region of the genome linked to a mutation and candidate lesions that may be causal for the phenotype of interest. We show that our method successfully identifies zebrafish mutations that cause nonsense or missense changes to codons, alter transcript splicing, or alter gene expression levels. Furthermore, we develop an online accessible or downloadable bioinformatics pipeline allowing for easy implementation of all steps of the method. Overall, we show that RNA-Seq is a fast, reliable, and cost effective method to map and identify mutations that will greatly facilitate the power of forward genetics in vertebrate models.
Publication Title
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Sample Metadata Fields
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View Samples- Danio rerio
- 1 Downloadable Sample
- IlluminaHiSeq2000
Description
Gene expression profiling of several wildtype strains of zebrafish embryos. The samples were pooled from several developmental stages ranging from 2 to 7 days post fertilization. This breadth of sampling gives a broad idea of genes expressed during early development and SNPs associated with wildtype strains.
Publication Title
RNA-seq-based mapping and candidate identification of mutations from forward genetic screens.
Sample Metadata Fields
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