Urechis unicinctus, a benthic marine worm inhabiting widely in the coast of Russia, Japan, Korean Peninsula and China, present special biological characteristics in morphology, reproductive and developmental biology as well as physiology. It could exist in condition of low oxygen, high sulfide and pollution where most animals could not live. However, the molecular mechanisms of resistance are still unknown and the main obstacle to further study is the limited genomic and genetic information. In order to improve the situation, we performed and acquired its transcriptome database.
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View SamplesUrechis unicinctus, a benthic marine worm inhabiting widely in the coast of Russia, Japan, Korean Peninsula and China, presents special biological characteristics in morphology, reproductive and developmental biology as well as physiology. It could exist in condition of low oxygen, high sulfide and pollution where most animals could not live.However, the molecular mechanisms of resistance are still unknown and the main obstacle to further study is the limited genomic and genetic information.
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View SamplesThe goal of this study is to investigate the genes regulated by histone acetylation during root development.
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Specimen part
View SamplesTo study the splicing factor role of ZmGRP1
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Sex, Specimen part, Disease, Disease stage, Cell line, Treatment
View SamplesThe Ptf1a gene has essential functions during several stages of pancreas development. It is expressed in the nave endoderm and required pancreas cell fate specification; it is also required later in the differentiation and maintenance of acinar cells. To identify the regulatory genetic program downstream of Ptf1a required for early pancreatic fate acquisition, we used microarrays to perform a comprehensive gene expression analysis of Ptf1a overexpressing endodermal tissue at NF32 and NF36.
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Specimen part
View SamplesNgn3 is a master regulator of pancreatic endocrine development. It is necessary for the creation of all endocrine cells in mice. Little is known about the genes that act downstream of the transcription factor Ngn3 in pancreas endocrine development to specify each of the endocrine lineages. As a consequence, little is known about the genes involved in early development and the specification of the beta cell. We used microarrays to identify Ngn3 downstream genes that are involved in early and ectopic beta cell development in Xenopus laevis. We overexpressed Ngn3 in the Xenopus early endoderm and analyzed the genes that are upregulated four hours after.
Transient expression of Ngn3 in Xenopus endoderm promotes early and ectopic development of pancreatic beta and delta cells.
Specimen part, Treatment
View SamplesThis study provide a dynamic atlas of endosperm development from Shaanxi within China
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Specimen part
View SamplesCuticular wax is a major composition of plant surface cuticle, which exerts crucial functions in optimizing plant growth. Histone acetylation regulates gene expression of diverse biological processes, but little is known about its role in cuticular wax synthesis. Here we observed that mutation of Arabidopsis histone acetyltransferase GCN5 gene impaired stem cuticular wax accumulation. Furthermore, three target genes of GCN5 (CER3, CER26 and CER1-L1) were identified by RNA-Seq and ChIP assays. Consistently, H3K9/14 acetylation levels in promoter regions of CER3, CER26 and CER1-L1 were significantly decreased in gcn5 as compared to wild type. Notably, overexpression of CER3 in the gcn5 mutant restored the stem cuticular wax accumulation. Collectively, these data demonstrate that Arabidopsis GCN5 is involved in stem cuticular wax accumulation by modulating CER3 expression via H3K9/14 acetylation, which present evidence that histone acetylation plays an important role in cuticular wax biosynthesis
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Specimen part
View SamplesWe used a chicken RNA microarray to identify differentially expressed genes in order to compare two layer lines kept in a small group housing system Eurovent Deutsch.
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Specimen part
View SamplesUsing HiSeq2000 to sequence white leghorn different parts (ovary, magnum, isthmus and uterus) of oviduct at 40-weeks.
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