Identification of circular RNAs in zebrafish
Identification and characterization of circular RNAs in zebrafish.
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View Samplesthe mRNA expression of pig lung tissue. there are four groups: LW pigs infected with PCV2, LW pigs control, Yorkshire × Landrace (YL) pigs infected with PCV2, Yorkshire × Landrace (YL) pigs control.
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Sex, Age, Specimen part, Disease, Disease stage, Cell line, Treatment
View SamplesSkeletal muscle is heterogeneous in nature and distinguished as red muscle and white muscle because of their myofiber composition. Soleus (SOL) is a typical red muscle and extensor digitorum longus (EDL) is a typical white muscle. In this study, we compared the transcriptome difference of soleus and extensor digitorum longus from three 10-week-old Yorkshire boars with porcine Affymetrix microarray.
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View SamplesLymphocyte count is one of the lymphocyte phenotypes that are related or potentially related to the individual anti-virus capacity in pigs and other mammals. The aim of this work is to compare the transcriptome of the poly I:C-induced peripheral blood cells between pigs with extreme values of total lymphocyte count.The pigs with the two-tailed critical value of lymphopenia post 4h with poly I:C stimulation from a Duroc-Erhualian F2 population.
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Specimen part, Compound
View SamplesNeutrophils play a key role in the innate immunity and the first line of defense against invading pathogens. The aim of this work is to investigate the neutrophil responses and transcriptomic alteration in the porcine peripheral blood by employing Poly I:C to emulate viral infection. The pigs with the two-tailed critical value of neutrophilia post 4h with poly I:C stimulation from a Duroc-Erhualian F2 population.
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Age, Specimen part, Subject, Compound
View SamplesThe transcriptome sequencing reveals the divergence of the genetic mechanism of reproductive traits in two Chinese native breeds. XH chicken was meat-type breed with low reproduction ability, with a 70~80% incidence of broodiness in population, with the duration of 15~30 d brooding, and with a production of 60~90 eggs per year. BEH chicken was layer-type breed with high reproduction ability, with a 10%~15% incidence of broodiness in population, with the duration of 7~20 d brooding, and with a production of 180 eggs per year.
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Sex, Specimen part
View SamplesTranscriptome of connective tissue of swine affected and non-affected with scrotal hernia
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Sex, Age, Specimen part, Disease
View SamplesThe equine hoof is a specialized structure in which the distal skeleton is suspended within the capsule by interdigitated structures known as laminae. Inflammation of this tissue, known as laminitis, is a devastating disease that is the second leading cause of both lameness and euthanasia in the horse. Current research on the laminitic transcriptome focuses on the expression of known genes. However, as this tissue is quite unique and equine annotation is largely derived from computational predictions and gene models from other species, there are likely yet uncharacterized transcripts expressed in the laminae that may be involved in the etiology of laminitis. In order to create a novel annotation resource, we performed whole transcriptome sequencing of sagittal lamellar sections from one control and two laminitis affected horses. Assembly of 113 million 100bp reads resulted in around 75,000 transcripts. Of these, 36,000 corresponded to known annotation in NCBI's non-redundant protein database. RT-PCR of 12 selected annotations confirmed structure and expression in lamellar tissue. Transcriptome sequencing represents a powerful tool to expand on equine annotation and identify novel targets for further laminitis research.
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View SamplesPorcine alveolar macrophages (PAMs) play an important role in innate immunity. Streptococcus suis serotype 2 is a pathogen responsible for several diseases in both pigs and humans. We used microarrays to study the transcriptome of PAMs infected with SS2.
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Age, Specimen part, Disease, Disease stage, Treatment
View SamplesExamination of whole genome gene expression profiles from the placentas of cloned (somatic cell nuclear transfer, SCNT) and normally produced (control) calves using RNA-seq.The differentially expressed genes were analyzed between SCNT and control placentas.
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Sex
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