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Homo sapiens
4 Downloadable Samples
Illumina HiSeq 2500
Description
RNA-Seq analysis of intestinal integrity-relevant genes in Caco-2 cell line exposure to aflatoxins M1 and ochratoxin A
Publication Title
No associated publication
Sample Metadata Fields
Sex, Age, Specimen part, Disease, Cell line, Race
View Samples- Gallus gallus
- 45 Downloadable Samples
- Illumina HiSeq 2500
Description
The main objective of this study was to decipher the gene expression profile associated with the onset and early development of Wooden Breast Disease in commercial broiler chickens. To achieve this, pectoralis major muscle biopsy samples were harvested from high-breast-muscle-yield, purebred broiler line. The pectoral biopsy samples were collected from the cranial (week 2 and 3) and caudal (week 4) aspects of the muscle belly in birds which were allowed to grow up to 7 weeks of age. Three subsets of biopsy samples comprising 6 unaffected (U) and 10 affected (A) from week 2 and 4 and 4U and 12A from week 3 were processed for RNA-sequencing analysis. All selected samples were processed using Truseq stranded mRNA LT sample kit for paired-end 2x75-nucleotide sequencing with Illumina Hiseq 2500 sequencer.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Age, Specimen part, Disease
View Samples- Gallus gallus
- 33 Downloadable Samples
- Illumina HiSeq 2500
Description
No description.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Age, Specimen part, Cell line, Treatment
View Samples- Sus scrofa
- 7 Downloadable Samples
- Illumina Genome Analyzer II
Description
The ability to mature oocytes in vitro provides a tool for creating embryos by parthenogenesis, fertilization and cloning. Unfortunately the quality of oocytes matured in vitro falls behind that of in vivo matured oocytes. To address this difference transcriptional profiling by deep sequencing was conducted on pig oocytes that were either matured in vitro or in vivo. Alignment of over 18 million reads identified 1,316 transcripts that were differentially represented. One pathway that was overrepresented in the oocytes matured in vitro was for Wingless-type MMTV integration site (WNT) signaling. In an attempt to inhibit the WNT pathway Dickkopf-related protein 1 was added to the in vitro maturation medium. Addition of Dickkopf-related protein 1 improved the percentage of oocytes that matured to the metaphase II stage, increased the number of nuclei in the resulting blastocyst stage embryos, and in oocytes reduced the amount of disheveled segment polarity protein 1 protein. It is concluded that transcriptional profiling is a powerful method for detecting differences between in vitro and in vivo matured oocytes, and that the WNT signaling pathway is important for proper oocyte maturation.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Age, Specimen part, Disease, Disease stage, Cell line, Treatment
View Samples- Ovis aries
- 8 Downloadable Samples
- Illumina HiSeq 2500
Description
To screening for gene expression differences between the ovaries of Baimei mutton with double-lambs and single-lamb using RNA-Seq method
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part
View Samples- Sus scrofa
- 6 Downloadable Samples
Affymetrix Porcine Genome Array (porcine)
Description
Gene differential expression in different pregnant periods and different tissues are detected.
Publication Title
No associated publication
Sample Metadata Fields
Specimen part, Time
View Samples- Sus scrofa
- 23 Downloadable Samples
- Affymetrix Porcine Genome Array (porcine)
Description
This study was performed to determine the effects of dietary fat sources, i.e., beef tallow, soybean oil, olive oil and coconut oil (each 3% in feed), on the growth performance, meat quality and gene expression in growing-finishing pigs.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part
View Samples
SRP167033- Sus scrofa
- 9 Downloadable Samples
- Illumina HiSeq 2500
Description
To evaluate the potential protective effects of APS on intestinal health and its mechanism of action, we performed an RNA sequencing (RNA-seq) study in LPS-stimulated porcine intestinal epithelial cells (IPEC-J2) in vitro
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part, Cell line
View Samples- Ovis aries
- 40 Downloadable Samples
- Affymetrix Bovine Genome Array (bovine)
Description
Expression profiling of sheep born to Australian industry sires with high and low genetic merit (Estimated Breeding Values or EBVs) for eye muscle depth (EMD). Progeny (40) from six Poll Dorset sires representing well defined extremes of EBVs for Eye Muscle Depth (low EBV EMD and high EBV EMD) were selected for analysis. The six sires were Australian industry sires with three sires representative of low EBV EMD and three representing high EBV EMD.
Publication Title
An Always Correlated gene expression landscape for ovine skeletal muscle, lessons learnt from comparison with an "equivalent" bovine landscape.
Sample Metadata Fields
No sample metadata fields
View Samples- Ovis aries
- 15 Downloadable Samples
- Affymetrix Bovine Genome Array (bovine)
Description
Expression data from Sheep longissimus dorsi (LD) muscle during development; fetal lambs (80, 100, 120 days gestation), new born lambs at birth (150 d) and lambs at 12 weeks (230 d)
Publication Title
A gene network switch enhances the oxidative capacity of ovine skeletal muscle during late fetal development.
Sample Metadata Fields
No sample metadata fields
View Samples