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Mus musculus
109 Downloadable Samples
Illumina HiSeq 2500
Description
No description.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part, Cell line, Treatment
View Samples- Mus musculus
- 35 Downloadable Samples
- Illumina HiSeq 2500
Description
RNAseq of YAC128 mice treated with pridopidine
Publication Title
No associated publication
Sample Metadata Fields
Sex, Age, Specimen part, Cell line, Treatment
View Samples- Zea mays
- 31 Downloadable Samples
- NextSeq 500
Description
FACS RNAseq of transgenic lines pWUS and pYAB
Publication Title
No associated publication
Sample Metadata Fields
Specimen part
View Samples- Caenorhabditis elegans
- 25 Downloadable Samples
- Illumina HiSeq 2000
Description
Raw sequence reads are provided for RNA-seq of parental and transgenerational worms in which the P0 were treated with OP50 (control) or PA14.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part, Cell line
View Samples- Caenorhabditis elegans
- 23 Downloadable Samples
- Illumina HiSeq 2000
Description
Identifying transcriptional changes in adults, whose biology and behavior differsubstantially from developing animals, is important when evaluating adult phenotypes.Moreover, cell- and tissue-specific information is critical for understanding the biologyof multicellular animals. We used adult cell-specific isolation to identify thetranscriptomes of C. elegans'' major adult tissues (muscle, intestine, epidermis, andneurons).
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part, Cell line
View Samples- Mus musculus
- 6 Downloadable Samples
- Illumina HiSeq 2500
Description
We evaluated the therapeutic activity of the modified U1 particles in a mouse model affected by severe spinal muscular atrophy. ExSpeU1 introduced by germline transgenesis efficiently rescued the phenotype increasing SMN2 exon 7 splicing, SMN protein production and radically extending the life span.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Age, Specimen part, Disease, Cell line
View Samples- Homo sapiens
- 4 Downloadable Samples
- IlluminaGenomeAnalyzerII
Description
Regulatory T-cells (Treg) play an essential role in the negative regulation of immune answers by developing an attenuated cytokine response that allows suppressing proliferation and effector function of T-cells (CD4+ Th). The transcription factor FoxP3 is responsible for the regulation of many genes involved in the Treg gene signature. Its ablation leads to severe immune deficiencies in human and mice. Recent developments in sequencing technologies have revolutionized the possibilities to gain insights into transcription factor binding by ChiP-Seq and into transcriptome analysis by mRNA-Seq. We combine FoxP3 ChiP-Seq and mRNA-Seq in order to understand the transcriptional differences between primary human CD4+ T helper and regulatory T-cells, as well as to study the role of FoxP3 in generating those differences. We show, that mRNA-Seq allows analyzing the transcriptomal landscape of T-cells including the expression of specific splice variants at much greater depth than previous approaches, whereas 50% of transcriptional regulation events have not been described before by using diverse array technologies.
Publication Title
Next-generation insights into regulatory T cells: expression profiling and FoxP3 occupancy in Human.
Sample Metadata Fields
No sample metadata fields
View Samples- Mus musculus
- 180 Downloadable Samples
- Illumina HiSeq 2500
Description
Mice carrying embryos that were either Pax6fl/fl;Emx1CreER (experimental group) or Pax6fl/+;Emx1CreER (control group) were given tamoxifen on embryonic day 9.5 (E9.5) to induce Pax6 deletion. Embryos were harvested on E13.5, the cerebral cortices were removed and divided into rostral and caudal halves, and total RNA was extracted. Samples from littermates of the same genotype were pooled. Poly-A mRNA was purified and TruSeq RNA-Seq libraries were prepared and sequenced (100 base paired-end; Illumina, HiSeq v3).
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples- Rattus norvegicus
- 172 Downloadable Samples
Affymetrix Rat Genome 230 2.0 Array (rat2302)
Description
Global expression profiling of epileptogenesis has been confounded by variability across laboratories, epilepsy models, tissue sampled and experimental platforms, with the result that very few genes demonstrate consistent expression changes. The present study minimizes these confounds by combining Affymetrix microarray datasets from seven laboratories, using three status epilepticus (SE) models of epilepsy in rats (pilocarpine, kainate, self-sustained SE or SSSE) and the rat kindling model. Total RNA was harvested from laser-captured dentate granule cells from 6 rats at three times during the early-to-mid latent phase that precedes epilepsy symptoms in the SE models (1, 3 and 10 days after SE), or 24 hr after the first stage 2, stage 4 and stage 5 seizure in the kindling model. Each epilepsy model was studied in two independent laboratories except SSSE. The initial goals of this study were to a) identify model-independent transcriptional changes in dentate granule cells that could point to novel intervention targets for epileptogenesis, b) characterize the basal transcriptional profile of dentate granule cells, and c) identify genes that have highly variable expression.
Publication Title
No associated publication
Sample Metadata Fields
Specimen part
View Samples
GSE4412- Homo sapiens
- 169 Downloadable Samples
- Affymetrix Human Genome U133A Array (hgu133a)
Description
Diffuse infiltrating gliomas are the most common primary brain malignancy found in adults, and Glioblastoma multiforme, the highest grade glioma, is associated with a median survival of 7 months. Transcriptional profiling has been applied to 85 gliomas from 74 patients to elucidate glioma biology, prognosticate survival, and define tumor sub-classes. These studies reveal that transcriptional profiling of gliomas is more accurate at predicting survival than traditional pathologic grading, and that gliomas characteristically express coordinately regulated genes of one of four molecular signatures: neurogenesis, synaptic transmission, mitotic, or extra-cellular matrix. Elucidation of these survival associated molecular signatures will aid in tumor prognostication and define targets for future directed therapy.
Publication Title
Gene expression profiling of gliomas strongly predicts survival.
Sample Metadata Fields
Sex, Age, Specimen part, Disease stage
View Samples