In this study, we purified periodontal ligament cell clones committed to osteoblastic/cementoblastic phenotype (C-O clones) and clones committed to fibroblastic phenotype (C-F clones), and employed RNA-seq to describe the differential transcriptional profile of osteoblastic/cementoblastic and fibroblastic cell clones from human periodontal ligament. The understanding of differences in periodontal ligament subpopulations can help to elucitade the favorable to formation of mineralizing and non-mineralizing tissues of periodontium.
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Sex, Age, Specimen part
View SamplesIn this study, we purified periodontal ligament cell clones committed to osteoblastic/cementoblastic phenotype (C-O clones) and clones committed to fibroblastic phenotype (C-F clones), and employed RNA-seq to describe the differential transcriptional profile of osteoblastic/cementoblastic and fibroblastic cell clones from human periodontal ligament. The understanding of differences in periodontal ligament subpopulations can help to elucitade the favorable to formation of mineralizing and non-mineralizing tissues of periodontium.
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Sex, Age, Specimen part, Treatment
View SamplesEarly embryo RNA-seq sequencing
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Sex, Specimen part
View SamplesCell wall modifications in response to low temperature compensation
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Specimen part, Treatment
View Sampleswe aimed to calculate the expression level of some special genes
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View SamplesSenescence has tremendous impact on yield and nutritional quality of agricultural produce. However, scant information is available on genetic architecture of senescence in maize (Zea mays L.) despite the importance of stay-green, a delayed senescence phenotype, in this major cereal crop. We combined different approaches including co-expression networks derived from time-course transcriptome analysis of senescence and stay-green to identify unique candidate genes.
No associated publication
Specimen part
View SamplesDetermination of the genes that can be important for the defense of the host to infection for Mucorales fungi, using zebrafish and the fungus Mucor circinelloides as host and pathogen model, respectively. Total RNA was sequenced (RNA-seq) from abdominal organs of infected fish.
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Sex, Specimen part, Cell line, Treatment
View SamplesThis SuperSeries is composed of the SubSeries listed below.
The Methyl-CpG-Binding Protein Mbd2 Regulates Susceptibility to Experimental Colitis via Control of CD11c<sup>+</sup> Cells and Colonic Epithelium.
Specimen part
View SamplesMice deficient in MBD2 (Mbd2-/-) were treated with 2% dextran sulfate sodium or normal drinking water for 6 continuous days. A single cell suspension of colon lamina propria and epithelium was isolated, with monocytes (CD11b+ Ly6CHi, MHC-II+/-), macrophages (CD11b+ Ly6C-MHC-II+), cDC2s (CD11b- CD11c+ CD103+) and epithelial cells (CD45- EpCAM+) purified by FACS.
The Methyl-CpG-Binding Protein Mbd2 Regulates Susceptibility to Experimental Colitis via Control of CD11c<sup>+</sup> Cells and Colonic Epithelium.
Specimen part
View SamplesT follicular helper cells (TFH) are heterogenic population of CD4+ T cells, expressing CXCR5+ and PD-1+ on their surface. Their role is linked to supporting formation of germinal centres (GC) and these cells are thought to express high levels of PD-1 marker. Two models of immunisation were used to investigate the role of PD-1 low TFH. In Salmonella enterica infection high frequency of T follicular helper cells expressing low levels of PD-1 surface molecule are observed within first week of infection but GC do not appear until much a later stage (week 7-8). Sheep red blood cell immunisation (SRBC) gives rise to both TFH and GC B cells within first week of response and these TFH express low to high level of PD-1 molecule.
No associated publication
Sex, Specimen part
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