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Arabidopsis thaliana
1 Downloadable Sample
Illumina HiSeq 2500
Description
to compare the transcriptome between WT and skl1 mutant
Publication Title
No associated publication
Sample Metadata Fields
Specimen part
View Samples
SRP160431- Mus musculus
- 12 Downloadable Samples
- Illumina HiSeq 2500
Description
C2C12 cells, as mouse-derived myoblasts, are a classic cell model for studying skeletal muscle development. We knocked down the immunoglobulin superfamily containing leucine-rich repeat (Islr) gene in C2C12 cell line and studied the effect of Islr on the proliferation and differentiation of C2C12 cells.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part, Cell line
View Samples- Danio rerio
- 7 Downloadable Samples
Affymetrix Zebrafish Genome Array (zebrafish)
Description
Effect of ethanol or nicotine exposure on gene expression compared to control. Duplicate arrays from ethanol or nicotine treated animals compared with triplicate arrays from paired control animals. In total 4 treatment arrays (2 ethanol, 2 nicotine) and 3 control arrays (from control animals treated in parallel with ethanol-treated fish and nicotine-treated fish.)
Publication Title
Gene expression changes in a zebrafish model of drug dependency suggest conservation of neuro-adaptation pathways.
Sample Metadata Fields
Specimen part, Compound
View Samples- Arabidopsis thaliana
- 31 Downloadable Samples
- NextSeq 500, Illumina HiSeq 2500
Description
This study was designed to understand the mechanism by which floral organ abscission mutants'' phenotypes arise.
Publication Title
No associated publication
Sample Metadata Fields
Specimen part, Treatment
View Samples- Mus musculus
- 8 Downloadable Samples
- NextSeq 500
Description
Neuro-2a cells were infected with ZIKV (MOI = 0.5) for 48 h and total RNA was extracted and purified using TRI Reagent and RNeasy Mini kit (Qiagen). RNA-seq was performed at the Molecular and Genomics Core Facility of the University of Mississippi Medical Center. Differential expression analysis between mock and ZIKV infected cells was done using Tophat and cuffdiff programs from the tuxedo suite.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part, Disease, Cell line
View Samples- Anopheles gambiae
- 2 Downloadable Samples
- Illumina HiSeq 2000
Description
RNAseq from male testes
Publication Title
Odorant receptor-mediated sperm activation in disease vector mosquitoes.
Sample Metadata Fields
Sex, Specimen part
View Samples- Arabidopsis thaliana
- 8 Downloadable Samples
- Illumina HiSeq 2500
Description
It has been reported that IRE1-bZIP60 pathway is conserved for the signal transduction and gene regulation in response to ER stress including high temperature among human, yeast, Arabidopsis, rice and maize. However, the information of their counterparts in wheat is limited and, especially, the biological relevance of the equivalent pathway contributing to heat tolerance is still ambiguous. In present study, we identified heat stress-induced wheat bZIP60 and investigated its splicing pattern depending on IRE1.
Publication Title
No associated publication
Sample Metadata Fields
Specimen part, Treatment
View Samples- Zea mays
- 1 Downloadable Sample
- Illumina HiSeq 2500
Description
In this study, the immature embyros of inbred line Z31and hybrid HiII were heated in 45 ?C water bath for 2 min, and then infected with Agrobacterium suspension for 5 min. After infection, the immature embryos were transferred onto CO-medium with or without 100 mg L-1 cysteine which is freshly prepared and filter-sterilized. After culturing for 3 days at 25 ?C under dark conditions, the immature embryos were collected, and stored at -70 ?C for RNA extraction. The genes up-regulated and down regulated both in Z31 and HiII were analyzed, and elucidate the role of cysteine in improving the efficiency of Agrobacterium infection of maize immature embryo, and provide important information for the improvement of the maize transformation.
Publication Title
No associated publication
Sample Metadata Fields
Specimen part
View Samples- Zea mays
- 1 Downloadable Sample
- Illumina HiSeq 2000
Description
In this study, the immature embyros of inbred line Z31and hybrid HiII were heated in 45 C water bath for 2 min, and then infected with Agrobacterium suspension for 5 min. After infection, the immature embryos were transferred onto CO-medium with or without 100 mg L-1 cysteine which is freshly prepared and filter-sterilized. After culturing for 3 days at 25 C under dark conditions, the immature embryos were collected, and stored at -70 C for RNA extraction. The genes up-regulated and down regulated both in Z31 and HiII were analyzed, and elucidate the role of cysteine in improving the efficiency of Agrobacterium infection of maize immature embryo, and provide important information for the improvement of the maize transformation.
Publication Title
No associated publication
Sample Metadata Fields
Specimen part
View Samples- Zea mays
- 1 Downloadable Sample
- Illumina HiSeq 2000
Description
In this study, the immature embyros of inbred line Z31and hybrid HiII were heated in 45 C water bath for 2 min, and then infected with Agrobacterium suspension for 5 min. After infection, the immature embryos were transferred onto CO-medium with or without 100 mg L-1 cysteine which is freshly prepared and filter-sterilized. After culturing for 3 days at 25 C under dark conditions, the immature embryos were collected, and stored at -70 C for RNA extraction. The genes up-regulated and down regulated both in Z31 and HiII were analyzed, and elucidate the role of cysteine in improving the efficiency of Agrobacterium infection of maize immature embryo, and provide important information for the improvement of the maize transformation.
Publication Title
No associated publication
Sample Metadata Fields
Specimen part
View Samples