to identify regulated genes in response to cytokinin in wild-type and 35S:ARR7 plants using the Affymetrix ATH1 full genome array.
Genome-wide expression profiling of ARABIDOPSIS RESPONSE REGULATOR 7(ARR7) overexpression in cytokinin response.
Disease, Disease stage, Compound, Time
View SamplesThe Columbia (Col-0) ecotype of Arabidopsis thaliana was used as wild type. lbd16, lbd18 single and lbd16 lbd18 double mutants were used as mutants.
No associated publication
Time
View SamplesThe experiment was designed to enable comparison between Columbia and ahk2/ahk3, ahk3/ahk4 double and ahk2/ahk3/ahk4 triple mutants Arabidopsis seedlings
No associated publication
Age, Time
View SamplesThe iaa1 mutant protein impaired a variety of auxin responses by acting as a negative regulator of auxin-responsive pathway.
Genome-wide analysis of the auxin-responsive transcriptome downstream of iaa1 and its expression analysis reveal the diversity and complexity of auxin-regulated gene expression.
Age, Compound, Time
View SamplesCHD3 proteins are ATP-dependent chromatin remodeling factors that are components of diverse multisubunit complexes that can either repress or activate gene expression. In plants, the CHD3 protein PICKLE (PKL) is necessary for repression of seed-specific genes during germination and promotes deposition of the repressive epigenetic mark trimethylation of histone H3 lysine 27 (H3K27me3). It is unknown, however, if PKL acts directly at H3K27me3-enriched loci. We undertook a microarray analysis of 14-day-old plants and found that PKL continues to play an important role in expression of H3K27me3-enriched genes and in specification of developmental identity after germination.
No associated publication
Specimen part
View SamplesMicroarrays revolutionized biological research by enabling gene expression comparisons on a transcriptome-wide scale. Microarrays, however, do not estimate absolute expression level accurately. At present, high throughput sequencing is emerging as an alternative methodology for transcriptome studies. Although free of many limitations imposed by microarray design, its potential to estimate absolute transcript levels is unknown.
Estimating accuracy of RNA-Seq and microarrays with proteomics.
Sex, Age
View SamplesThe research of maize freezing tolerance.
No associated publication
Specimen part
View SamplesComparing the gene expression patterns between wild type plant (Col-0) and MYB Over-expression plants.
Omics-based identification of Arabidopsis Myb transcription factors regulating aliphatic glucosinolate biosynthesis.
No sample metadata fields
View SamplesNucellar embryony is a form of apomixis found in citrus where somatic nucellar cells differentiate into embryos and are included in the seed resulting from the normal sexual process. The nucellar cells giving rise to adventive embryo start proliferating prior to anthesis and fully differentiate obtaining nourishment from sexually derived endosperm. To identify transcripts differentially expressed during nucellar embryo initiation we have taken RNA samplesfrom different developing stages of ovules from polyembryonic (cv. Vaniglia Sanguigno) and monoembryonic (cv. Temple) cultivars. We used microarray for a detailed analysis of global gene expression during nucellar embryony initiation and development. We have further validated the differentially expressed genes using qRT-PCR.
No associated publication
Specimen part
View SamplesWhole genome transcriptome profiling of bulked RILs with high and low grain number per panicle derived from 2 cultivars at panicle primordia stage
No associated publication
No sample metadata fields
View Samples