Stresses that target mitochondrial function lead to altered transcriptional responses for 100-1000s of genes genome wide, and are signalled via retrograde signalling pathways within the cell. rao1 mutants contain a mutation in a gene encoding a Cyclin-Dependant Kinase E;1 and cannot induce stress responsive genes (such as the mitochondrial alternative oxidase 1a) in response to mitochondrial dysfunction. We sought to define the global gene network regulated through RAO1 function in response to mitochondrial stress (mimicked through treatment of plants with antimycin A - a specific inhibitor of complex III in the mitochondrial electron transfer chain). We have defined global stress responses that are positively and negatively mediated by RAO1 function, as well as global stress responses to antimycin A treatment that are regulated independently of RAO1.
Cyclin-dependent kinase E1 (CDKE1) provides a cellular switch in plants between growth and stress responses.
Age, Specimen part, Treatment
View SamplesTranscript abundance profiles were examined over the first 24 hours of germination in rice grown under aerobic conditions.
Experimental analysis of the rice mitochondrial proteome, its biogenesis, and heterogeneity.
Specimen part, Time
View SamplesEffect of high light on directly exposed and shaded, distal Arabidopsis leaf tissue
Systemic and intracellular responses to photooxidative stress in Arabidopsis.
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View SamplesFlies (Drosophila melanogater) of 4 strains (M: Oregon-R, D: Dark-fly, U: Urbana-S, R: RAL-774) were reared in three light conditions (1, 4, 7: constant light; 2, 5, 8: light/dark cycling; 3, 6, 9: constant dark). Total RNAs were extracted from whole bodies of adult females. RNA expression was compared between strains and between light conditions to reveal effects of genome x environment interaction.
No associated publication
Sex, Specimen part, Cell line
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