Inducible overexpression of Arabidopsis meristem regulators by AlcR / AlcA system. Plants harboring 35S::AlcR/AlcA::GOI (GUS control, LEAFY, SHOOTMERSTEMLESS, WUSCHEL)constructs were grown in continous light for 12 days and induced with 1% Ethanol. After 12h of EtOH treatment, seedlings were dissected and RNA was processed from the shoot apex and young leaves. Affymetrix Ath1 arrays were hybridized in duplicates from each experiment.
WUSCHEL controls meristem function by direct regulation of cytokinin-inducible response regulators.
Age, Specimen part, Subject, Compound
View SamplesArabidopsis etiolated seedlings (4d old) Col-0 wild type compared to det3 mutants under various growth conditions
Reduced V-ATPase activity in the trans-Golgi network causes oxylipin-dependent hypocotyl growth Inhibition in Arabidopsis.
Age
View SamplesComparison of Arabidopsis seedlings with disturbed function of CDKB2;1 and CDKB2;2 by either overexpression or knock-down
Requirement of B2-type cyclin-dependent kinases for meristem integrity in Arabidopsis thaliana.
Specimen part
View SamplesIdentification of Hox gene downstream genes at embryonic stages 11 and 12<br></br><br></br>Functional diversification of body parts is dependent on the formation of specialized structures along the various body axes. In animals, region-specific morphogenesis along the anterior-posterior axis is controlled by a group of conserved transcription factors encoded by the Hox genes. Although it has long been assumed that Hox proteins carry out their function by regulating distinct sets of downstream genes, only a small number of such genes have been found, with very few having direct roles in controlling cellular behavior. We have quantitatively identified hundreds of Hox downstream genes in Drosophila by microarray analysis, and validated many of them by in situ hybridizations on loss- and gain-of-function mutants. One important finding is that Hox proteins, despite their similar DNA binding properties in vitro, have highly specific effects on the transcriptome in vivo, as expression of many downstream genes responds primarily to a single Hox protein. In addition, a large fraction of downstream genes encodes realizator functions, which directly affect morphogenetic processes, such as orientation and rate of cell divisions, cell-cell adhesion and communication, cell shape and migration, or cell death. Focusing on these realizators, we provide a framework for the morphogenesis of the maxillary segment. Since the genomic organization of Hox genes and the interaction of Hox proteins with specific cofactors are conserved in vertebrates and invertebrates, and similar classes of downstream genes are regulated by Hox proteins across the metazoan phylogeny, our findings represent a first step towards a mechanistic understanding of morphological diversification within a species as well as between species.
Comparative analysis of Hox downstream genes in Drosophila.
Age, Time
View SamplesAbove ground tissue of 10 day old Arabidopsis seedlings of Col wild-type, 35S-ARR7, arr7, 35S-ARR15 was treated with Cytokinin (benzyladenine), Auxin (indole-3-acetic acid) or both.
Hormonal control of the shoot stem-cell niche.
Age, Specimen part, Treatment
View Samplescomparison of Landsberg errecta wild-type with slm mutants in apex and flowers
DETORQUEO, QUIRKY, and ZERZAUST Represent Novel Components Involved in Organ Development Mediated by the Receptor-like Kinase STRUBBELIG in Arabidopsis thaliana
Age, Specimen part
View SamplesResponse of control (flc-3) and dominant late flowering mutant (smz-D flc-3) to inductive photoperiod (tissue: apices)
No associated publication
Age, Specimen part, Time
View SamplesComparison of expression profiles between greenworms and wildtype looking plants
A triplet expansion associated genetic defect in Arabidopsis thaliana
Age, Specimen part, Disease, Disease stage, Subject
View SamplesResponse of Arabidopsis control (flc-3) and a dominant repressor of flowering (smz-D flc-3) to inductive photoperiod
No associated publication
Age, Specimen part, Time
View SamplesAfter zygote division, the resulting daughter cells progressively give rise to two very different tissue types. With the use of microarrays, global nuclear expression profiles were generated.
Cell type-specific transcriptome analysis in the early Arabidopsis thaliana embryo.
Specimen part
View Samples