To identify genes associated with the heat stress (HS) memory, transcript profiling using Affymetrix ATH1 microarrays was performed to compare Col-0 seedlings after the priming stimulus with control plants (unprimed).
The plastid metalloprotease FtsH6 and small heat shock protein HSP21 jointly regulate thermomemory in Arabidopsis.
Specimen part
View SamplesGlobal transcriptome patterns were determined in XVE-14 and wild-type seedlings induced for 45 min b-estradiol in order to identify the genes early regulated by EBE transcription factor.
EBE, an AP2/ERF transcription factor highly expressed in proliferating cells, affects shoot architecture in Arabidopsis.
Specimen part
View SamplesTo understand the effect of high and low GA levels on plant metabolism and development in Arabidopsis we made use of the GA biosynthesis inhibitor paclobutrazol (PBZ) and exogenously applied GA. The whole genome response at the translation level was assessed by immunopurification of polysomes from PBZ- and GA treated plants expressing FLAG-tagged ribosomal protein L18 (RPL18B). Polysomal associated RNA was isolated and subjected to affymterix ATH1 CHIP analysis. A total of 140 genes were statistically determined to be differentially translated after GA treatment whereas 89 genes where affected PBZ treatment. Our analysis revealed that GA and PBZ have opposing effects on the expression of cell wall and wax layer biosynthesis related genes. In addition, many genes involved in secondary metabolism are upregulated upon PBZ treatment. A set of SAUR-like genes important for mediating auxin responses are downregulated by PBZ, which is of interest to coordinatian of GA levels with growth and development. Interestingly, GA treatment induces the upregulation of transcription factors related to plant defense and senescence, which is in agreement with the early flowering upon GA treatment. Our study provides a first picture of the response of Arabidopsis to altered GA levels at the translation level, and thus will be valuable for understanding gene regulation at the polysome level.
Translatome and metabolome effects triggered by gibberellins during rosette growth in Arabidopsis.
Specimen part, Treatment
View SamplesThe aim of the experiment was to identify genes rapidly responding at their expression level to enhanced expression of the transcription factor GRF9.
GROWTH-REGULATING FACTOR 9 negatively regulates arabidopsis leaf growth by controlling ORG3 and restricting cell proliferation in leaf primordia.
Age, Specimen part
View SamplesGlobal transcriptome patterns were performed using SHYG-IOE-5h (5h after Estradiol and Mock treatment)
NAC transcription factor speedy hyponastic growth regulates flooding-induced leaf movement in Arabidopsis.
Specimen part, Treatment
View SamplesThis SuperSeries is composed of the SubSeries listed below.
General detoxification and stress responses are mediated by oxidized lipids through TGA transcription factors in Arabidopsis.
No sample metadata fields
View Samples12-oxo-phytodienoic acid (OPDA) and phytoprostane A1 (PPA1) are cyclopentenone oxylipins that are formed via the enzymatic
General detoxification and stress responses are mediated by oxidized lipids through TGA transcription factors in Arabidopsis.
No sample metadata fields
View SamplesGlobal transcriptome patterns were obtained from ATAF1-IOE seedlings at 1 h, 2 h and 5 h after estradiol induction or mock treatment, and from mature ATAF1-IOE leaves at 5 h after estradiol induction or mock treatment.
Transcription Factor Arabidopsis Activating Factor1 Integrates Carbon Starvation Responses with Trehalose Metabolism.
Specimen part, Treatment, Time
View Samples12-Oxo-phytodienoic acid (OPDA) and several phytoprostanes are structurally related cyclopentenone oxylipins that can be formed via the enzymatic jasmonate pathway and a non-enzymatic, free radical-catalyzed pathway, respectively. To elucidate the biological activities of phytoprostanes in comparison to OPDA as well as the metabolism we performed genome-wide expression analysis.
General detoxification and stress responses are mediated by oxidized lipids through TGA transcription factors in Arabidopsis.
No sample metadata fields
View SamplesTo identify genes that are regulated by SERF1, we performed expression profiling on roots of serf1 and wild-type plants under standard growth conditions.
SALT-RESPONSIVE ERF1 is a negative regulator of grain filling and gibberellin-mediated seedling establishment in rice.
Specimen part
View Samples