Transcriptome of Prmt5-deficient and control mouse activated B cells

Quiescent splenic B cells purified from Cg1-cre Prmt5F/F cells and Cg1-cre control mice. Resting B cells were plated on feeder cells expressing CD40L and BAFF and supplemented with IL-4 for activation. Four days later, the resulting activated germinal center-like B cells were purified and RNA extracted and processed for HiSeq. Four independent samples of each genotype were processed and analyzed. Overall design: 2 Experiments, 2 samples of each genotype per experiment (Exp 1: Samples 1,2,7,8 ; Exp 2: Samples 3,4,5,6)_ PRMT5 FF Cg1cre: Samples 1,2,3,4_ Cg1cre controls: Samples 5,6,7,8

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Litzler LC, Zahn A, Meli AP, Hébert S, Patenaude AM, Methot SP, Sprumont A, Bois T, Kitamura D, Costantino S, King IL, Kleinman CL, Richard S, Di Noia JM