Impact of Tcf1 and Lef1 deficiency on follicular helper T (Tfh) cells

Comparison of transcriptome between control and Tcf1/Lef1-deficient germinal center Tfh (GC-Tfh) cells Overall design: Control mice or those are deficient for Tcf1 and Lef1 transcription factors were infected with vaccinia virus. On day 8 after infection, the splenocytes were isolated and surface stained to identify CD44-high, CD62L-low, GFP+, CD4+ T cells. From these cells, PD-1+, CXCR5+ GC-Tfh cells were sorted for RNAseq analysis

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Choi YS, Gullicksrud JA, Xing S, Zeng Z, Shan Q, Li F, Love PE, Peng W, Xue HH, Crotty S