Identification of IL-1 and IL-6-responsive genes in human monocyte-derived macrophages

Using whole-genome Affymetrix microarrays (HG-U133A), we characterized the transcriptome profile of cultured human macrophages stimulated for 4 h with interleukin 1 (IL-1) or interleukin 6 (IL-6). We found that, in distinction to liver cells, IL-1 is much more potent than IL-6 in modifying macrophage gene expression, although considerable heterogeneity in response of macrophages deriving from individual blood donors was observed. The obtained results permitted to identify a large number of cytokine-responsive genes. coding for proteins of unknown function that are now being studied in our laboratory. They may represent novel targets in the anti-inflammatory therapy.

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Jura J, Wegrzyn P, Korostyński M, Guzik K, Oczko-Wojciechowska M, Jarzab M, Kowalska M, Piechota M, Przewłocki R, Koj A