Comparative transcriptome analysis of yeast expressing the fungal desaturases

To study how the presence of PUFAs influences central cellular processes, and in order to perform lipidome, transcriptome and molecular studies we decided to use yeast as a model organism. We therefore co-expressed 12-desaturase and 6- desaturase genes from Mucor rouxii in S. cerevisiae with the objective to obtain a yeast strain that contains PUFAs, especially linoleic acid (LA, C18:29,12) and -linolenic acid (GLA, C18:36,9,12), in its membranes.

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Ruenwai R, Neiss A, Laoteng K, Vongsangnak W, Dalfard AB, Cheevadhanarak S, Petranovic D, Nielsen J