Transcription profiling of hMC cells treated with PDGF (10ng/ml) LXA4 (1nM) for a 24h period

Previous data have demonstrated the attenuation of platelet-derived growth factor (PDGF)-induced hyperproliferation and migration in primary human mesangial cells by lipoxin A4. In these experiments we aimed to find out what the effect of Lipoxin A4 would be on the global genomic changes associated with PDGF. We treated cells with PDGF (10ng/ml) ± LXA4 (1nM) for a 24h period and examined genomic differences, we could derive from our results that lipoxin A4 was diminishing the pro-inflammatory, pro-proliferative and pro-fibrotic responses induced by PDGF.

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