Transcription profiling of skeletal muscle from wild type and Six1/ Six4 knock out mice at E10.5 to identify genes under the control of the Six proteins

The aim of the experiment was to compare the transcriptome of Six1-/-Six4-/- and control embryos in order to identify genes under the control of Six proteins at E10.5.<br></br><br></br>E10.5 embryos were eviscerated, head and limbs were discarded, the neural tube was removed, and RNAs were prepared with the remaining axial tissues. <br></br><br></br>E10.5 RNAs from three SixdKO and two control embryos were hybridized on Affymetrix mouse genome 430A2.0 arrays (Affymetrix, Strasbourg - France).

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